论文部分内容阅读
为明确镰刀菌毒素在小麦中的污染与分布状况,选取以四种镰刀菌毒素的特异性抗体制备的ELISA试剂盒,检测了来自全国范围内的183个冬小麦、46个春小麦品种(系)样品中的镰刀菌毒素含量,并对不同麦区小麦样品中脱氧雪腐镰刀菌烯醇(DON)、伏马(fumonisin)、单端孢霉烯醇(T-2)和玉米赤霉烯酮(ZON)四种毒素含量及污染率进行比较分析。结果表明,我国小麦品种(系)中镰刀菌毒素污染比较普遍,但污染水平较低,均符合国家规定标准。四种镰刀菌毒素中以DON毒素为主,其平均含量为73.87μg/kg。不同麦区间DON、fumonisin和T-2毒素的含量存在显著差异(P<0.01),而ZON毒素的含量在麦区间差异不显著(P>0.05),东北春麦晚熟组、长江中下游冬麦组和黄淮冬麦区南片冬水组镰刀菌毒素总体含量较高。DON与ZON毒素污染率在麦区间存在显著差异(P<0.01),而fumonisin与T-2毒素污染率差异不显著(P>0.05)。在不同麦区间,东北春麦晚熟组的DON毒素的含量和污染率均为最高。
In order to clarify the contamination and distribution of Fusarium toxins in wheat, ELISA kits prepared from four Fusarium toxins-specific antibodies were selected and tested from 183 winter wheat and 46 spring wheat varieties (lines) Fusonin, trichothecene (T-2), and zearalenone (T-2) in wheat samples from different wheat regions ZON) four kinds of toxins content and pollution rate comparative analysis. The results showed that the contamination of Fusarium mycotoxins was common in wheat cultivars (lines) in our country, but the pollution level was low, which met the national standard. The four kinds of Fusarium toxin to DON toxins, the average content of 73.87μg / kg. There were significant differences in the contents of DON, fumonisin and T-2 toxin between different wheat cultivars (P <0.01), but no significant differences in the contents of ZON toxin between wheat cultivars (P> 0.05) And Huanghuai winter wheat area south piece of winter water Fusarium toxins higher overall levels. There was a significant difference in DON and ZON toxin contamination rates in wheat (P <0.01), but no significant differences in fumonisin and T-2 toxin contamination (P> 0.05). In different wheat regions, the content of DON toxin and the contamination rate of northeast spring wheat late maturing group were the highest.