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目的分析ELISA法在诊断梅毒螺旋体感染中的临床检验效果。方法 80例疑似梅毒螺旋体感染患者,所有患者均分别进行酶联免疫吸附法(ELISA)以及甲基胺红不加热血清试验(TRUST)检测,比较两种检测方法阳性诊断正确率。结果 80例疑似患者经TRUST法检测后,敏感性为84.91%(45/53),特异性为44.44%(12/27),阳性诊断正确率为75.00%(45/60);80例疑似患者经ELISA法检测后,敏感性为96.67%(58/60),特异性为60.00%(12/20),阳性诊断正确率为96.67%(58/60),两种检测方法阳性诊断正确率比较差异具有统计学意义(χ~2=11.5820,P<0.05)。结论梅毒螺旋体感染患者在临床中选择ELISA方法进行诊断,效果显著,可为临床检验提供科学依据。
Objective To analyze the clinical effect of ELISA in the diagnosis of Treponema pallidum infection. Methods Eighty patients with suspected infection with Treponema pallidum were enrolled in this study. All patients were tested by ELISA and TRUST, and the diagnostic accuracy of the two tests was compared. Results The sensitivity and specificity of 80 suspected cases were 84.91% (45/53) and 44.44% (12/27), respectively. The positive rate of positive diagnosis was 75.00% (45/60) in 80 cases of suspected patients The sensitivity and specificity were 96.67% (58/60) and 60.00% (12/20) respectively after the ELISA. The positive diagnostic accuracy was 96.67% (58/60). The positive diagnostic accuracy of the two methods were compared The difference was statistically significant (χ ~ 2 = 11.5820, P <0.05). Conclusion Treponema pallidum infection in patients with clinical choice of ELISA method for diagnosis, the effect is significant, can provide a scientific basis for clinical testing.