目的:探明影响反应器内铁皮石斛原球茎生长及物质合成的因素,为铁皮石斛原材料的大量生产提供一种新方法。方法:利用3 L气升式生物反应器,以组培原球茎为材料,研究了接种量、光照强度和通气量对原球茎增殖生长和多糖及石斛碱积累的影响。结果:当接种量为10 g.L-1时,培养30 d后原球茎颜色深绿,生长健壮。多糖含量在接种量处理间无差异,但石斛碱含量有差异(接种量10 g.L-1处理最高),多糖和石斛碱生产量在10 g.L-1接种密度中最高。光照强度1 600lx条件下原球茎生长最旺盛,光照对铁皮石斛原球茎多糖的积累起到促进作用,但对石斛碱的积累则有抑制作用,多糖含量和生产量在1 600,2 400 lx光照处理下好于暗条件处理;石斛碱的含量虽然在暗处理中最高,但因原球茎生长不佳,石斛碱的生产量在1 600 lx的光照强度处理中出现最大值。通气量为0.2(空气体积/培养体积/min)时原球茎生长健壮,颜色鲜绿,生长优于0.1,0.3处理,且多糖和石斛碱含量和产量均达最大值。结论:在工作体积为2 L的气升式球型生物反应器内,接入10 g.L-1原球茎外植体,光照强度调节为1 600 lx,通气量为0.2有利于原球茎生长和多糖及石斛碱的生产,铁皮石斛原球茎生物反应器培养是大量快速生产多糖和石斛碱的有效途径。 Objective: To find out the factors that affect the protocorm growth and material synthesis in Dendrobium officinale, and to provide a new method for the mass production of Dendrobium officinale. Methods: The effects of inoculum size, light intensity and aeration volume on the proliferation of protocorms and the accumulation of polysaccharides and dendroboside were studied using 3 L airlift bioreactor. Results: When the inoculum size was 10 g.L-1, the protocorms became dark green and grew robustly after 30 days of culture. Polysaccharide content in the inoculum size was no difference, but there was a difference in the content of dendrobin (inoculation 10 g.L-1 highest treatment), polysaccharide and dendrobhin production in 10 g.L-1 inoculation density highest. Light intensity of 1 600lx protocorm growth under the most vigorous, light on the protocorm-like Dendrobium protocorm polysaccharide accumulation has played a catalytic role, but the accumulation of germ plasm is inhibited, polysaccharide content and production at 1600 and 2400 lx light Treatment under dark conditions than treatment; although the content of dendrobin in dark treatment highest, but due to poor growth of protocorms, the production of dendrobin in 1600 lx light intensity treatment in the maximum. Protocorm grew robustly and had a bright green color when the aeration volume was 0.2 (air volume / culture volume / min), and its growth was better than that of 0.1 and 0.3. The content of polysaccharide and dendrobin and the yield reached the maximum. Conclusion: In the airlift type spherical bioreactor with a working volume of 2 L, the introduction of 10 gL-1 protocorm explants with a light intensity of 1 600 lx and aeration of 0.2 is conducive to the growth of protocorms and polysaccharides And dendroboid production, protocorm-like protocorm biological cultivation of Dendrobium candidum is a large number of effective ways to quickly produce polysaccharides and dendrobin.