论文部分内容阅读
目的建立树鼩单纯疱疹病毒(Herpes Simplex Virus,HSV)感染模型,为研究HSV潜伏感染机制、病毒与宿主相互作用的表观遗传学机制提供基础数据。方法先在不同细胞系中比较HSV-1 17+毒株的感染效果,然后用HSV-1 17+毒株通过眼膜划痕的方法感染树鼩,观察其发病情况及死亡率;采集树鼩脑组织进行PCR检测,并采血检测HSV-1抗体。结果 HSV-1 17+毒株可感染多种细胞并引起细胞发生病变;树鼩感染死亡率约为33.33%,树鼩脑组织采用PCR均检测到HSV-1基因组;采用ELISA检测树鼩感染HSV-1后血清特异性抗体阳性率偏低,2周和3周时分别为4.55%和5.56%。结论成功建立树鼩HSV-1感染模型,在感染动物组织中检测到HSV-1DNA,部分感染动物血清中检测到HSV-1IgG抗体,为HSV相关研究奠定基础。
Objective To establish a herpes simplex virus (HSV) infection model and provide the basic data for studying the latent infection mechanism of HSV and the epigenetic mechanism of virus-host interaction. Methods The HSV-1 17+ strains were compared in different cell lines. The tree shrews were infected with HSV-1 17+ strain through the scalp. The incidence and mortality of HSV-17 + Brain tissue was detected by PCR and blood was collected to detect HSV-1 antibody. Results The HSV-1 17+ strain could infect a variety of cells and cause cell lesions. The mortality rate of tree gall infection was about 33.33%. The HSV-1 genome was detected by PCR in tree shrews. HSV-1 was detected by ELISA. The positive rate of serum specific antibody was low at 4.5 and 5.56% at 2 weeks and 3 weeks respectively. Conclusion The HSV-1 infection model was successfully established in the tree shrews. HSV-1 DNA was detected in the infected animal tissues. HSV-1 IgG was detected in the sera of partially infected animals, which laid the foundation for HSV-related studies.