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目的:探讨大肠癌血管内皮生长因子(VEGF)的表达,微血管生成及其与临床病理参数的关系。方法:采用组织芯片及S-P免疫组化法应用VEGF多克隆抗体、CD34多克隆抗体,检测30例大肠腺癌及10例正常大肠组织中VEGF的表达,计数微血管密度(MVD)。结果:大肠癌中VEGF表达阳性率明显高于正常的大肠黏膜,分别为90%和20%,P<0.05。大肠癌中的MVD明显高于正常大肠黏膜,分别为:59.8±8.7和18.2±1.7,P<0.05。VEGF阳性者MVD显著高于VEGF阴性者,VEGF表达率和MVD与大肠癌Dukes分期、浸润深度密切相关,P<0.05。结论:VEGF表达与大肠癌血管生成有关,大肠癌的发生发展依赖血管生成。MVD可作为大肠癌的预后因素。
Objective: To investigate the expression of vascular endothelial growth factor (VEGF), angiogenesis and its relationship with clinicopathological parameters in colorectal cancer. Methods: VEGF polyclonal antibody and CD34 polyclonal antibody were used to detect the expression of VEGF in 30 cases of colorectal adenocarcinoma and 10 cases of normal colorectal cancer by tissue microarray and S-P immunohistochemistry. The microvessel density (MVD) was counted. Results: The positive rate of VEGF expression in colorectal cancer was significantly higher than that in normal colorectal mucosa (90% and 20%, P <0.05). The MVD in colorectal cancer was significantly higher than that in normal colorectal mucosa (59.8 ± 8.7 and 18.2 ± 1.7, P <0.05). The positive rate of MVD in VEGF-positive patients was significantly higher than that in VEGF-negative patients. The expression of VEGF and MVD were closely related to Dukes stage and depth of invasion in colorectal cancer (P <0.05). Conclusion: The expression of VEGF is related to angiogenesis of colorectal cancer. The occurrence and development of colorectal cancer depend on angiogenesis. MVD can be used as a prognostic factor for colorectal cancer.