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目的探索胃壁注射A型肉毒毒素(BTX-A)对大鼠摄食量、体重和ghrelin、NPY表达的影响及治疗肥胖症的机制。方法将72只大鼠随机分为3批(2周、6周、12周),每批24只再分为4组:对照组(Control),胃窦注射BTX-A组(Antrum),胃底体注射BTX-A组(Fundus),全胃多点注射BTX-A组(Multi-site),每组6只。观察大鼠摄食量、体重,血浆ghrelin、NPY浓度,胃ghrelin、下丘脑NPY免疫组化;胃ghrelin-mRNA、下丘脑NPY-mRNA表达。结果与对照组比较,BTX-A组大鼠的摄食量和体重均下降,ghrelin和NPY在血浆和组织中的表达减低,上述改变Multi-site组>Fundus组>Antrum组(P<0.05);BTX-A注射后2、6、12周,四组间差异性逐渐减小。结论胃壁注射BTX-A可通过下调脑肠肽ghrelin和NPY的表达,使大鼠摄食减少、体重减轻;这可能是BTX-A应用于肥胖症治疗的机制之一。
Objective To investigate the effects of botulinum toxin type A (BTX-A) on the ingestion, body weight, ghrelin and NPY expression in rats and the mechanism of treatment of obesity. Methods Thirty-two rats were randomly divided into three groups (2 weeks, 6 weeks and 12 weeks). Each group of 24 rats was divided into 4 groups: Control group, Antrum, The basal body was injected with BTX-A group (Fundus), and the BTX-A group (Multi-site) was injected into the whole stomach with 6 rats in each group. The food intake, body weight, plasma ghrelin, NPY concentration, gastric ghrelin, hypothalamic NPY immunohistochemistry; gastric ghrelin-mRNA, hypothalamus NPY-mRNA expression were observed. Results Compared with the control group, the food intake and body weight of rats in BTX-A group decreased and the expression of ghrelin and NPY in plasma and tissues decreased. The changes of Multi-site group> Fundus group> Antrum group (P <0.05); At 2, 6, 12 weeks after BTX-A injection, the differences among the four groups gradually decreased. Conclusion BTX-A injection into the stomach wall can reduce the ingestion and weight loss of ghrelin and NPY, which may be one of the mechanisms for the treatment of obesity.