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目的 :研究三七总皂甙和银杏叶提取物对急性氧中毒的预防作用及其可能机制。方法 :分别给小鼠连续腹腔注射三七总皂甙和银杏叶提取物 5d后 ,在 5 0 0kPa高压氧中暴露 6 0min ,观察惊厥潜伏期、惊厥次数、惊厥间隔时间等指标 ;另外测定高压氧暴露 15min后脑组织中活性氧单位、脂质过氧化物、一氧化氮、谷胱甘肽的含量和过氧化氢酶、谷胱甘肽过氧化物酶、单胺氧化酶的活性。结果 :三七总皂甙和银杏叶提取物可以明显延长氧惊厥潜伏期和惊厥间隔时间 ,减少惊厥次数 ;降低高压氧暴露后脑组织中脂质过氧化物、一氧化氮的含量 ,使活性氧单位、谷胱甘肽含量和谷胱甘肽过氧化物酶活性保持在较高的水平 ;对过氧化氢酶和单胺氧化酶活性的影响则不显著。结论 :三七总皂甙和银杏叶提取物可以有效预防急性氧中毒 ,其机制可能与它们的抗氧化活性有关。
Objective : To study the preventive effect of panax notoginseng saponins and Ginkgo biloba extract on acute oxygen toxicity and its possible mechanism. METHODS: Mice were given intraperitoneal injections of panax notoginseng saponins and Ginkgo biloba extract for 5 days, exposed to 500 psig of hyperbaric oxygen for 60 min, and the indices of convulsive latency, number of convulsions, and time of convulsions were measured. In addition, hyperbaric oxygen exposure was measured. After 15 min, the contents of reactive oxygen species, lipid peroxides, nitric oxide, and glutathione, and the activities of catalase, glutathione peroxidase, and monoamine oxidase in brain tissue. Results: Panax notoginseng saponins and Ginkgo biloba extract can significantly prolong the latency of oxygen convulsion and seizure duration, reduce the number of convulsions, and reduce the content of lipid peroxide and nitric oxide in the brain tissue after exposure to hyperbaric oxygen, making the active oxygen units, Glutathione content and glutathione peroxidase activity remained at high levels; the effect on catalase and monoamine oxidase activity was not significant. Conclusion: Panax notoginseng saponins and Ginkgo biloba extract can effectively prevent acute oxygen poisoning. The mechanism may be related to their antioxidant activity.