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目的 :探讨肽能神经纤维进入垂体前叶的途径及神经纤维的功能。方法① :经咽旁入路切断大鼠垂体柄 ,术后 8d观察垂体前叶内CGRP免疫反应阳性纤维密度的变化。结果 :切断垂体柄术后一周垂体前叶内约 80 %的CGRP免疫反应阳性纤维消失 ,提示至少绝大部分的CGRP免疫反应纤维经过垂体柄进入垂体前叶 ,残留纤维是溃变不完全抑或是另有来源尚不清楚。方法② :暴露大鼠垂体柄。切除或保留肾上腺 ,保温静置 60min后局麻垂体柄 ,股动脉放血刺激ACTH分泌。结果 :①切除肾上腺并局麻垂体柄大鼠 ,急性失血后 5min内ACTH水平低于对照组 ,在 3min时间点差异显著 (P <0 .0 5 )。保留肾上腺组大鼠局麻垂体柄后对失血的应激反应亦呈同样趋势。结论 :上述结果提示垂体柄内可能存在兴奋性纤维促进急性失血后ACTH的快速分泌反应 ,但该作用可能被迅速增强的CRH作用逐渐掩盖 ;②急性失血后各组大鼠垂体前叶POMCmRNA变化不明显。
Objective: To investigate the pathway of nerve fibers into the anterior pituitary and the function of nerve fibers. Method ①: The paravertebral approach was used to cut off the pituitary stalk of rats, and the changes of CGRP immunoreactive fiber density in the anterior pituitary were observed 8 days after operation. Results: About 80% of CGRP immunoreactive fibers disappeared in the anterior pituitary after the pituitary stalk was transected, suggesting that at least the majority of CGRP immunoreactive fibers enter the anterior pituitary via the pituitary stalk and the residual fiber is incompletely or Another source is not clear. Method ②: Exposed rat pituitary stalk. Excision or retention of the adrenal, heat stand for 60min after local anesthesia pituitary stalk, femoral artery to stimulate ACTH secretion. Results: ①The level of ACTH in 5 min after acute blood loss in adrenalectomized rats was lower than that in control group. There was a significant difference at 3 min (P <0.05). Retaining the adrenal gland pituitary stalks in rats after the response to blood loss also showed the same trend. CONCLUSION: The above results suggest that there may be excitatory fibers in the pituitary stalk to promote the rapid secretion of ACTH after acute blood loss, but this effect may be gradually masked by the rapid enhancement of CRH. ② After acute blood loss, the changes of POMC mRNA in the anterior pituitary of rats obvious.