从R3 克隆中提取并纯化流行性出血热R2 2 株M片段cDNA ,用光敏生物素标记而制备出生物素化的cDNA探针。用此探针与R2 2 株、76 118株及R3 克隆进行斑点杂交 ,均出现阳性杂交信号 ,而与仙台病毒、鼠肺炎病毒及BALB/c小鼠肝细胞、Vero E6细胞和SP2 / 0细胞杂交均为阴性。探针的灵敏度为 2 5pg目的核酸。用此探针测定了来自 9个单位 12株人用鼠源性杂交瘤细胞和 7个单抗制剂均为阴性。该结果与血清学检验结果一致 Extract and purify the M genomic cDNA of R2 strain R2 from R3 clones and prepare the biotinylated cDNA probe by biotin labeling. Positive hybridization signals were observed with dot blot hybridization with R2 2, 76 118 and R3 clones, but not with Sendai virus, murine pneumovirus and BALB / c mouse hepatocytes, Vero E6 cells and SP2 / 0 cells Hybrids are negative. The sensitivity of the probe is 25 pg of target nucleic acid. Using this probe, 12 human murine hybridoma cells from 9 units and 7 monoclonal antibody preparations were tested negative. This result is consistent with the serological test results