采用不同的加热时间和溶液酸度提取苜蓿叶蛋白 ,对苜蓿叶蛋白凝集量和苜蓿叶蛋白中粗蛋白含量进行测定。结果表明 :提取苜蓿叶蛋白的加热时间在 7～ 9min较为适宜 ,提取时溶液pH值应当控制为 3.5。苜蓿叶蛋白中粗蛋白含量的变化不大 ,在 5 4%～ 6 5 %之间 Different heating time and solution acidity were used to extract alfalfa leaf protein, and the content of alfalfa leaf protein and crude protein in alfalfa leaf protein were determined. The results showed that the heating time of extracting alfalfa leaf protein was suitable at 7 ~ 9min, and the pH of the solution should be controlled at 3.5 when it was extracted. The content of crude protein in alfalfa leaf protein did not change much, between 54% and 65%