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在蛋白质中的谷氨酸和天门冬氨酸的电分析方法,目前只有单个测定方法,未见有连续测定方法的报道.本文研究了在浓度为0.020g/L谷氨酸和0.010 g/L天门冬氨酸,pH为3.5～4.5的硼酸体系中同时出现两灵敏度和分辨率均比较高的极谱波,峰电位分别为0.30V和0.60V,检出限分别为0.0050g/L和0.0025g/L,当其浓度分别为0.0100～0.1000g/L和0.005～0.05g/L与峰电流呈线性关系.浓度为0.500g/L胱氨酸、酪氨酸、亮氨酸、脯氨酸等中性氨基酸对测定无大的影响.回收率分别为95.0～100.1%和96.5～101.6%,未发现有系统误差存在.经3次对人发水解液分析,并与氨基酸分析仪测量结果对照,平均值分别为9.50%和9.55%,相对误差为1.50%和1.80%,均在国家规定的范围内.本法简便快速,灵敏度高,稳定性好,可用于各种蛋白质中的谷氨酸和天门冬氨酸的测定.
Currently, there are only single assay methods for the determination of glutamic acid and aspartic acid in proteins, and no continuous determination method has been reported.In this paper, we investigated the effects of glutamic acid and aspartic acid at concentrations of 0.020 g / L and 0.010 g / L Aspartic acid, pH3.5 ~ 4.5 boric acid system at the same time have two high sensitivity and resolution of the polarographic wave, the peak potentials were 0.30V and 0.60V, the detection limits were 0.0050g / L and 0.0025 g / L, when the concentration was 0.0100 ~ 0.1000g / L and 0.005 ~ 0.05g / L, respectively, the peak current was linear.The concentration of 0.500g / L cystine, tyrosine, leucine, proline And other neutral amino acids had no significant effect on the determination.The recoveries were 95.0 ~ 100.1% and 96.5 ~ 101.6%, respectively, and no systematic errors were found.After three times human hair hydrolyzate analysis and comparison with the amino acid analyzer measurement results , The average values were 9.50% and 9.55% respectively, the relative errors were 1.50% and 1.80%, all within the national regulations.The method was simple, rapid, sensitive and stable, and could be used for glutamic acid And aspartic acid determination.