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作者曾证明十全大补汤(TJ-48)能增强抗体生成、激活巨噬细胞并增强其吞噬活性。为阐明TJ-48促进抗体产生作用的机理,作者以脾细胞和淋巴细胞为主观察了TJ-48的促细胞分裂活性。TJ-48仍为热水抽出物的喷雾干燥粉末,生理盐水或RPMI1640溶解后用0.45μm微型过滤器滤过供实验用。实验用小鼠为雌性BALB/c、C_3H/HeJ以及BALB/c(nu/nu裸鼠)。无菌操作取小鼠脾脏、胸腺及淋巴结制成单个细胞悬液,除去红细胞后,用含10％胎牛血清及5×10~(-5)M 2-ME的RPMI1640培养液调整细胞数,加入96孔培养板中,每孔2×10~5个细
The authors have demonstrated that Shiquan Dabu Decoction (TJ-48) can enhance antibody production, activate macrophages and enhance its phagocytic activity. To elucidate the mechanism by which TJ-48 promotes the production of antibodies, the authors observed the mitogenic activity of TJ-48 by splenocytes and lymphocytes. TJ-48 is still a spray-dried powder of hot water extract. After the solution of physiological saline or RPMI1640 is dissolved, it is filtered through a 0.45 μm microfilter for experimental use. The experimental mice were female BALB/c, C_3H/HeJ, and BALB/c (nu/nu nude mice). Aseptically, mouse spleen, thymus, and lymph nodes were prepared into single cell suspensions. After red blood cells were removed, the number of cells was adjusted with RPMI1640 medium containing 10% fetal bovine serum and 5×10 −5 M 2-ME. Add 96-well plates, each hole 2 × 10 ~ 5 fine