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近来,水痘带状疱疹病毒(VZV)感染可以通过病毒分离、血清学、免疫荧光和凝胶扩散法进行实验室诊断。病毒分离最少需要2至3天;血清学诊断要求恢复期血清,需要更长时间;染色水疱液(VF)的免疫荧光法是快速的,但不能广泛适用;凝胶扩散法需要12～48小时,而且也不能广泛适用。相反,对流免疫电泳(CIE)适合于病毒和细菌感染的快速诊断,对VZV感染可在2小时之内作出诊断。取1%琼脂糖(用0.05M巴比妥缓冲液,pH8.6配制)4微升注于显微镜玻片上。作成相距8毫米,直径为2毫米的孔隙。往阴极孔隙注满VZV抗原或VF,往阳极孔隙
Recently, varicella zoster virus (VZV) infections have been laboratory diagnosed by virus isolation, serology, immunofluorescence and gel diffusion. Virus isolation requires at least 2 to 3 days; serological diagnosis requires convalescent serum that takes longer; immunofluorescence staining of blister fluid (VF) is rapid but not widely applicable; gel diffusion methods require 12 to 48 hours , But also can not be widely applied. In contrast, convective immunity electrophoresis (CIE) is suitable for the rapid diagnosis of viral and bacterial infections, and diagnosis of VZV infection can be made within 2 hours. Four microliters of 1% agarose (formulated with 0.05 M barbiturate buffer, pH 8.6) was placed on a microscope slide. Make pores 8 mm apart and 2 mm in diameter. VZV antigen or VF is filled into the cathode pores, toward the anode pores