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目的观察IL-6与上皮-间质转化(EMT)因子在胃癌组织表达情况相关性及其与病人预后的关系。方法采用免疫组化法检测上海交通大学医学院附属新华医院病理科2006年9月至2008年2月间60例手术切除胃癌组织和60例配对正常胃黏膜组织中IL-6,Twist,E-caherin及Vimentin蛋白的表达。结果胃癌组织中IL-6、Twist及Vimentin表达阳性率、E-cadherin表达阴性率均比正常胃黏膜组织中高,并与胃癌的分化程度、TNM分期、淋巴结转移情况、浸润深度以及生存期较短有显著相关性(P<0.05),与性别、年龄及肿瘤大小无关。IL-6的表达与Twist、Vimentin表达呈正相关,与E-cadherin表达呈负相关。结论 IL-6蛋白的表达与EMT相关蛋白、胃癌侵袭转移以及临床预后因素相关,可为胃癌侵袭转移的研究和病人的预后判断提供有价值的参考。
Objective To investigate the correlation between the expression of IL-6 and epithelial-mesenchymal transition (EMT) in gastric cancer and its relationship with prognosis. Methods Immunohistochemical method was used to detect the expression of IL-6, Twist, E-cadherin in 60 cases of gastric cancer tissues and 60 cases of paired normal gastric mucosa tissues from September 2006 to February 2008 in Department of Pathology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine. caherin and Vimentin protein expression. Results The positive rates of IL-6, Twist and Vimentin in gastric cancer tissues and the negative rates of E-cadherin expression in gastric cancer tissues were higher than those in normal gastric mucosa tissues, and were significantly correlated with the degree of differentiation, TNM stage, lymph node metastasis, depth of invasion and survival There was a significant correlation (P <0.05), regardless of sex, age and tumor size. The expression of IL-6 was positively correlated with the expression of Twist and Vimentin, but negatively correlated with the expression of E-cadherin. Conclusion The expression of IL-6 is related to EMT-related protein, invasion and metastasis of gastric carcinoma and clinical prognostic factors, which may provide valuable reference for the study of invasion and metastasis of gastric cancer and prognosis of patients.