论文部分内容阅读
目的 :观察肿瘤坏死因子 -a( TNF-a)和脂质结合唾液酸 ( LSA)在慢性肝病中的变化及其临床价值。方法 :以酶联免疫吸附法 ( EL ISA)和化学抽提法 ,分别定量检测 16例慢性肝炎、16例肝硬化和 75例肝癌患者血清 TNF -a和 L SA浓度 ,并进行了比较分析。结果 :慢性肝病患者血清 TNF和 L SA浓度均增加 ,明显高于正常对照组 ( P<0 .0 1)。在慢性肝炎、肝硬化和肝癌患者中 ,TNF-a异常率分别为 3 1.3 %、43 .8%和 93 .3 % ;L SA异常率分别为 2 5 .0 %、6 .3 %和 73 .3 % ;在肝癌患者中两者的总阳性率达 97.3 %。结论 :慢性肝病时肝细胞合成的 TNF-a和 L SA增加 ,它们的联合检测有助于肝癌的诊断和鉴别诊断
Objective: To observe the changes and clinical value of tumor necrosis factor-a (TNF-a) and lipid-associated sialic acid (LSA) in chronic liver disease. METHODS: Serum TNF-a and L SA concentrations in 16 patients with chronic hepatitis, 16 patients with liver cirrhosis, and 75 patients with liver cancer were quantified by enzyme-linked immunosorbent assay (EL ISA) and chemical extraction methods, respectively. Results: Serum TNF and LSA concentrations increased in patients with chronic liver disease, which was significantly higher than that in the normal control group (P<0.01). In patients with chronic hepatitis, cirrhosis, and liver cancer, the abnormal rate of TNF-a was 31.3%, 43.8%, and 93.3%, respectively; the abnormal rate of LSA was 25.0%, 6.3%, and 73% respectively. In the patients with liver cancer, the total positive rate of both cases was 97.3%. Conclusion :In chronic liver disease, the synthesis of TNF-a and L SA in hepatocytes is increased. Their combined detection is helpful for the diagnosis and differential diagnosis of liver cancer.