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应用生物素标记的p16cRNA探针、p16单克隆抗体，对42例人脑星形细胞瘤Ⅰ、Ⅱ、Ⅲ-Ⅳ级组织标本进行原位杂交和免疫组化研究。结果显示：p16mRNA原位杂交阳性信号呈蓝色或紫蓝色细颗粒状，位于胞浆内。Ⅰ、Ⅱ级杂交阳性细胞密度显著高于Ⅲ－Ⅳ级（P＜0．001），阳性细胞反应强度明显大于Ⅲ－Ⅳ级阳性强度。P16免疫组化阳性反应信号为棕黄色；Ⅰ和Ⅱ级免疫反应阳性细胞密度大于Ⅲ－Ⅳ级，（P<0．005，P<0．01）。p16原位杂交与P16免疫组化结果基本一致，二者呈正相关。研究结果提示，P16对星形细胞瘤的发生发展具有负向调节作用；p16在星形细胞瘤的恶性转化中也起到了重要作用。
Biotin-labeled p16cRNA probes and p16 monoclonal antibodies were used to perform in situ hybridization and immunohistochemical studies on 42 specimens of human astrocytomas I, II, and III-IV. The results showed that the positive signal of in situ hybridization of p16 mRNA was blue or purple blue fine granular and located in the cytoplasm. The density of I- and II-grade hybrid positive cells was significantly higher than that of III-IV (P<0.001). The intensity of positive cells was significantly greater than that of III-IV. The positive signal of P16 immunohistochemistry was brown-yellow; the density of positive I and II immune cells was greater than that of III-IV (P<0.005, P<0.01). The results of in situ hybridization of p16 and P16 immunohistochemistry were basically the same, and there was a positive correlation between the two. The results suggest that P16 has a negative regulatory effect on the development of astrocytoma; p16 also plays an important role in the malignant transformation of astrocytoma.