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目的比较脐血（ＣＢ）ＡＣ１３３＋细胞扩增前后ＣＤ３４＋ 细胞亚群表面归巢相关粘附分子ＶＬＡ－４（ＣＤ４９ｄ）、ＶＬＡ－５（ＣＤ４９ｅ）、ＬＦＡ－１（ＣＤ１１ａ）、Ｌ－ｓｅｌｅｃｔｉｎ（ＣＤ６２Ｌ）和ＰＥＣＡＭ－１（ＣＤ３１）等的表达情况，以评价细胞因子介导的体外扩增对干／祖细胞（ＨＳＰＣ）归巢功能的影响。方法将从新鲜ＣＢ标本中纯化的ＡＣ１３３＋ 细胞接种于无血清培养基ＱＢＳＦ－６０的无基质悬浮体系培养扩增１４ｄ，加入早期作用因子ＦＬ、ＳＣＦ和ＴＰＯ组合（ＦＳＴ），并在接种０ｄ时添加一剂ＩＬ－３，分别于培养０，７、１０和１４ｄ检测扩增潜能和上述几种粘附分子的表达情况。结果（１）在１４ｄ的培养扩增中，各阶段的ＨＳＰＣ均得到有效扩增，至１４ｄ时ＡＣ１３３＋和ＣＤ３４＋细胞分别增加３３．５０和６４．５６倍；（２）表达上述粘附分子的各ＣＤ３４＋ 细胞亚群均有不同程度（约２０～１６０倍）的扩增；（３）扩增后ＣＤ３４＋ 细胞表面的粘附分子ＣＤ１１ａ、ＣＤ４９ｅ和ＣＤ４９ｄ的表达与原代ＣＤ３４＋细胞持平或上升，而ＣＤ６２Ｌ和ＣＤ３１的表达则有不同程度的下调。结论我们建立的短期培养体系不仅可以支持ＣＢ ＨＳＰＣ的?
Objective To compare the expression of adhesion molecules VLA-4 (CD49d), VLA-5 (CD49e), LFA-1 (CD11a) and L-selectin (CD62L) on the surface of CD34 + cells before and after the expansion of cord blood AC133 + And PECAM-1 (CD31) in order to evaluate the effect of cytokine-mediated in vitro amplification on the homing function of stem / progenitor cells (HSPCs). Methods AC133 + cells purified from fresh CB specimens were inoculated into serum-free medium QBSF-60 and cultured for 14 days. The cells were treated with FL, SCF and TPO combinations (FST) A dose of IL-3, respectively, at 0, 7, 10 and 14 days to detect the amplification potential and the expression of several adhesion molecules. Results (1) During the 14 days of culture, the HSPCs in each stage were effectively expanded, and the numbers of AC133 + and CD34 + cells increased by 33.50 and 64.56 folds respectively at 14 days. (2) (3) The expression of adhesion molecules CD11a, CD49e and CD49d on the surface of CD34 + cells after amplification was the same as that of primary CD34 + cells, while CD62L And CD31 expression have different degrees of down regulation. Conclusion The short-term culture system that we established can not only support CB HSPC?