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作者应用布鲁氏菌深红色血培养基琼脂稀释技术,测定了三种新喹诺酮类药物司帕沙星(Sparfloxacin)、替马沙星和WIN57273对厌氧菌的抗菌活性。其它抗菌药物包括:环丙沙星、Imipenem、氯霉素、甲硝唑、头孢替坦、头孢西丁和羟氨苄青霉素加克拉维酸。由于研究是分别进行的,应避免直接对照,本文是三项研究的摘要。实验中使用的细菌是Wadsworth医疗中心最近临床分离的菌株中随机采集的,并按规定程序鉴定。MICs测定用10~5CFU的接种菌和布鲁氏菌深红色血培养基。平皿在GasPak瓶(BBL,Cockeysville,Md)中,或是一个厌氧室(Anaerobe Systems,San Jose,Calif)中孵育37℃48h。以细菌未生长,或未见单一
The authors used Brucella brucellosis blood agar dilution technique to determine the antibacterial activity of three new quinolones Sparfloxacin, Temafloxacin and WIN57273 against anaerobic bacteria. Other antimicrobials include: Ciprofloxacin, Imipenem, Chloramphenicol, Metronidazole, Cefotetan, Cefoxitin and Amoxicillin plus Clavulanic acid. Since the studies were conducted separately, direct controls should be avoided, and this article is a summary of three studies. The bacteria used in the experiment were randomly collected from the recently clinically isolated strains of Wadsworth Medical Center and identified according to established procedures. MICs were assayed with 10 to 5 CFU of inoculum and Brucella brucellosis blood medium. Plates were incubated at 37 ° C for 48 h in GasPak bottles (BBL, Cockeysville, Md) or in an anaerobic chamber (Anaerobe Systems, San Jose, Calif). With bacteria is not growing, or not seen in a single