论文部分内容阅读
烟草愈伤组织在再生过程中淀粉粒的数量是继代过程中的 8 2 2倍 ,淀粉酶活性为 4 4 8倍 ;胡萝卜中则无显著变化 ,分别为 0 95和 1 13倍。再生过程中 ,烟草愈伤组织中淀粉粒的数量、淀粉酶的活性分别为胡萝卜的 6 37和 6 14倍 ;继代过程中二者无显著差异 ,分别为 0 74和 1 55倍。烟草细胞中的淀粉粒被I2 KI染成蓝色 ,于质体中单个存在 ,在细胞内分布不均匀 ,常绕细胞核聚集 ;胡萝卜细胞中的淀粉粒被I2 KI染成紫色或红紫色 ,2～ 4个或更多个聚集于质体内 ,在细胞内基本均匀分布。对淀粉与植物细胞离体再生途径决定的关系进行了讨论。
The number of starch granules in tobacco callus during regeneration was 82 2 times higher than that in the subculture process, while the amylase activity was 448 times. There was no significant change in carrot, which was 0 95 and 113 times respectively. During regeneration, the amount of starch granules and the amylase activity in tobacco callus were 6 37 and 6 14 times higher than that of carrot, respectively. There was no significant difference between the two during callus induction, which was 0 74 and 1 55 times, respectively. Starch grains in tobacco cells were stained blue with I2 KI, present individually in the plastids, unevenly distributed within the cells and often clustered around the nucleus; starch granules in carrot cells were dyed purple or reddish purple with I2 KI; 2 ~ 4 or more aggregates within the plastid and is distributed substantially uniformly within the cell. The relationship between starch and plant regeneration pathway in vitro was discussed.