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MEAM1和MED是烟粉虱Bemisia tabaci两种重要的外来入侵隐种,在我国部分地区常混合发生,对我国农业生产造成了不同程度的危害和损失。尤其是MED隐种危害寄主范围更广,对多种杀虫剂具有较高抗性,防治上更为困难。因此,如何快速鉴定烟粉虱MEAM1和MED隐种,对于烟粉虱防治策略的选择具有十分重要的意义。本研究选择线粒体细胞色素氧化酶I(mitochondrial cytochrome oxidaseI,mtDNACOI)基因保守区域内的单核苷酸多态性(SNP)为靶标,应用等位基因聚合酶链式反应技术,借助TaqMan-MGB荧光染色标记探针,建立了一种鉴定烟粉虱MEAM1和MED隐种的等位基因选择性PCR方法,并对北京11个区县的14个烟粉虱种群进行了隐种鉴定。结果表明,北京地区14个烟粉虱种群样本与已知烟粉虱MED隐种种群在荧光值分布上聚为一簇,为MED隐种。该鉴定方法具备SNP基因分型的优点,可快速、可靠、高通量地鉴定烟粉虱MEAM1和MED,为烟粉虱隐种鉴定及遗传分化研究提供了新的可选途径。
MEAM1 and MED are two important invasive alien species of Bemisia tabaci, which are often mixed in some areas in China, causing different degrees of harm and loss to agricultural production in China. In particular, MED hidden dangers host a broader range of insecticides on a variety of high resistance, prevention and control more difficult. Therefore, how to quickly identify the white-shedding MEAM1 and MED hidden species is very important for the selection of the control strategy of Bemisia tabaci. In this study, single nucleotide polymorphisms (SNPs) in the conserved region of the mitochondrial cytochrome oxidase I (mtDNACOI) gene were selected as the targets. By using allele PCR and TaqMan-MGB fluorescence A marker-selective probe of MEAM1 and MED in B. tabaci was established to identify the allele-selective PCR of 14 populations of Bemisia tabaci in 11 districts and counties in Beijing. The results showed that the 14 populations of Bemisia tabaci in Beijing were clustered with the fluorescence distribution of the known MED populations of B. tabaci, which was a MED species. The identification method has the advantages of SNP genotyping and can be used to identify Bemisia tabaci MEAM1 and MED in a fast, reliable and high-throughput manner. The identification method provides a new alternative approach for identification and genetic differentiation of Bemisia tabaci.