Aim:To investigate differential gene expression and the pharmacological mecha-nism of baicalin and gardenin in focal cerebral ischemia in rats with high-densitycDNA microarray.Methods:Rat left middle cerebral arteries were occluded andtreated with either baicalin or gardenin.The pharmacological effects were inves-tigated using the difference in infarction areas before and after treatment,whichwere determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining.Gene ex-pression was demonstrated using a“Biostar40S”gene microarray.Semiquantitativereverse transcription-polymerase chain reaction (RT-PCR) was used to verify theresult of the selected genes.Results:Both baicalin and gardenin reduced theinfarction areas in focal cerebral ischemia rats (P<0.05).The differential geneswere 211,177,and 70 (upregulated or downregulated) in the model group,baicalin,and gardenin treatment groups compared with the sham-operated group,respectively.Gene expression of RpL19 and Csnk2 underwent an approximately1.9 and 2.1-fold increase,respectively,verified by semiquantitative RT-PCR,whichwas the same trend as the cDNA microarray.Conclusion:Differential gene ex-pression with respect to the pharmacological effects of baicalin and gardenin onfocal cerebral ischemia by cDNA microarray revealed a number of clues with re-spect to the therapeutic mechanisms of Chinese traditional medicine.In additionthe present study provided theoretical and experimental evidence that will aidfuture studies examining cerebral ischemia. Aim:To investigate differential gene expression and the pharmacological mecha-nism of baicalin and gardenin in the phase cued ischemia ischemia in rats with high-density cDNA microarray.Methods:Rat left middle cerebral arteries were occluded and treated with either baicalin or gardenin.The pharmacological effects were inves -tigated using the difference in infarction areas before and after treatment,whichwere determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining.Gene ex-pression was demonstrated using a“Biostar40S”gene microarray.Semiquantitativereversetranscription-polymerase chain Reaction (RT-PCR) was used to verify theresult of the selected genes.Results:Both baicalin and gardenin reduced the infarction areas in focal cerebral ischemia rats (P<0.05).The differential geneswere 211,177,and 70 (upregulated or downregulated) in the Model group,baicalin,and gardenin treatment groups compared with the sham-operated group,respectively.Gene expression of RpL19 and Csnk2 underwent an appro Ximately1.9 and 2.1-fold increase,respectively,verified by semiquantitative RT-PCR,whichwas the same trend as the cDNA microarray.Conclusion:Differential gene ex-pression with respect to the pharmacological effects of baicalin and gardenin onfocal cerebral ischemia by cDNA microarray Revealed a number of clues with re-spect to the therapeutic mechanisms of Chinese traditional medicine.In addition the present study provided theoretical and experimental evidence that it would aidfuture