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目的研究保健食品中黄芪甲苷的高效液相色谱测定方法。方法采用蒸发光散射检测器(ELSD)以黄芪甲苷为对照品,对保健食品中的黄芪甲苷进行高效液相色谱法分析,色谱柱:C18柱;流动相:乙睛∶水为15∶25;流速0.8 m l/m in,柱温40℃,漂移管温度100℃,气体压力3.0 Ba。结果按照选定的色谱条件进行测定,平均加标回收率为84.6%~92.6%,相对标准偏差为3.22%。结论本文建立的检测方法操作简便,可靠易行,可用于保健食品中黄芪甲苷的测定。
Objective To study the determination of astragaloside in health food by high performance liquid chromatography. Methods Evaporative light scattering detector (ELSD) was used for the determination of astragaloside Ⅳ in health food by HPLC. The column was C18 column, the mobile phase consisted of acetonitrile and water at 15: 25; flow rate 0.8 ml / m in, column temperature 40 ℃, drift tube temperature 100 ℃, gas pressure 3.0 Ba. The results were determined according to the selected chromatographic conditions. The average recoveries were 84.6% ~ 92.6% with relative standard deviations of 3.22%. Conclusion The detection method established in this paper is simple, reliable and easy to use and can be used for the determination of Astragaloside IV in health food.