神经胶质成熟因子(Glia maturationfactor,GMF)是1种酸性蛋白,可从生理pH值的成年脑组织中提取,易受多种蛋白酶的作用,不耐热,在高pH,以及变性剂的作用下均可使之失活。但其活性不受DNA酶、RNA酶或过氧化碘酸盐的影响;乙醇和丁醇等脂类提取剂亦不会使其活性消失。我们还发现在猪脑中有高分子和低分子两种类型的神经胶质成熟因子,前者的分子量为200,000,后者的分子量为40,000。在粗制脑提取液中,高分子量GMF占全部GMF生物活性的85％。在冰冻、融化 Glia maturation factor (GMF) is an acidic protein that is extracted from adult brain tissue at physiological pH and is susceptible to various proteases, heat labile, at high pH, ​​and the role of denaturants Under can make it inactivated. But its activity is not affected by DNase, RNase or peroxodisulfate; lipid extractants such as ethanol and butanol will not disappear its activity. We also found that there are two types of glial maturation factors, macromolecules and low molecules in the pig brain. The former has a molecular weight of 200,000 and the latter has a molecular weight of 40,000. In crude brain extracts, high molecular weight GMFs account for 85% of the total GMF bioactivity. In the frozen, thawed