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通常的分离纯化巨球蛋白血症患者血清单克隆IgM的方法常分为几步,产量低且常有α_2巨球蛋白的掺杂。作者对凝胶过滤法略加改进,得到了纯度高产量大的单克隆IgM。方法是在用Sephadex G200,Sepha crylS200和SephacrylS300凝胶柱进行分离时,将通常用0.05M PBS平衡和洗脱的方法改用0.005M PBS进行平衡,用含1.7MNaCl的0.05M PBS进行洗脱。用通常的方法分离含单克隆IgM的血清,可出现四个峰,IgM主要存在第一峰,其中有α2巨球蛋白掺杂。用改进的方法,也出现四个峰,第四峰明显增高,其中含IgM而不含IgG和α2
The usual method for isolating and purifying monoclonal IgM serum from patients with macroglobulinemia is often divided into several steps, with low yields and often doping with α 2 macroglobulin. The authors slightly modified the gel filtration method to obtain monoclonal IgM with high purity and high yield. The method is that, when separating by Sephadex G200, Sephacryl S200 and Sephacryl S300 gel columns, balance is usually carried out with 0.05M PBS and eluted with 0.005M PBS, and eluted with 1.7M NaCl containing 0.05M PBS. Separation of monoclonal IgM-containing sera by conventional methods yielded four peaks, with the primary peak of IgM, with alpha2 macroglobulin doping. With the improved method, there are also four peaks, the fourth peak was significantly higher, which contains IgM but not IgG and α2