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本文将构建的活化癌基因Ｔ２４－ｒａｓｃＤＮＡ的正、反义真核表达重组体，按先正义后反义和先反义后正义的不同转染顺序，转染ＮＩＨ３Ｔ３细胞，经药物筛选，建立细胞系。通过细胞形态及生物学特性观察、Ｓｏｕｔｈｅｒｎ杂交实验、软琼脂集落形成实验和Ｐ２１蛋白表达量的检测等实验对转化细胞进行了研究。结果表明：本室构建的正义重组体能诱导ＮＩＨ３Ｔ３细胞转化，而反义重组体可有效抑制这一转化，并且抑制效果与正、反义质粒转染的先后顺序有关。
The positive and antisense eukaryotic expression recombinants of the activated oncogene T24-rascDNA will be transfected into NIH3T3 cells according to different transfection sequences of sense antisense and antisense after sense antisense, followed by drug screening to establish cells. system. The transformed cells were studied by observation of cell morphology and biological characteristics, Southern hybridization experiments, soft agar colony formation experiments and detection of P21 protein expression. The results show that the sense recombinant construct constructed in this laboratory can induce the transformation of NIH3T3 cells, and the antisense recombinant can effectively inhibit this transformation, and the inhibitory effect is related to the order of transfection of antisense and antisense plasmids.