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1974年,我们同广西寄生虫病研究所合作,以周期型马来丝虫皮下感染长爪沙鼠获得成功,以后为获取较大数量的虫源,改用腹腔接种法。最初实验方法以腹腔穿刺获取阳性沙鼠腹腔液,针挑法收集3期幼,并以中华按蚊为媒介,实践中发现,腹腔穿刺抽液困难,一次接种常需准备数只种鼠,针挑法耗时长,易损伤幼虫,中华按蚊娇嫩不易饲养,蚊卵保存时间短,不利于保种。1979年以来,我们改用腹腔灌洗法获取微丝蚴,贝氐分离法收集3期幼,并以东乡伊蚊为媒介,提高了接种成功率。
In 1974, we cooperated with Guangxi Institute of Parasitic Diseases to successfully infect Cyclops gerbil (Meriones unguiculatus) subcutaneously with periodic malachite worms. In order to obtain a larger number of insect pests, we switched to intraperitoneal inoculation. The initial experimental method to obtain positive peritoneal dialysis rat gerbils, acupuncture pick collection of 3 young, and Chinese Anopheles as a medium, found in practice, paracentesis pumping difficulties, often need to prepare a vaccination only a few rats, needles Pick method takes a long time, easy to damage larvae, Chinese Anopheles delicate not easy to keep, mosquito eggs save a short time, is not conducive to maintaining seed. Since 1979, we switched to intraperitoneal lavage to obtain microfilariae. We collected the third instar larvae by the method of Begonia angustifolia, and used Aedes aegypti to increase the success rate of inoculation.