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为了研究高锌(Zn)胁迫下苹果砧木八棱海棠(Malus robusta Rehd.)Zn积累、分配及分子响应机制,以其8叶龄实生苗为试验材料,采用溶液培养法进行不同浓度(4、25、50、100μmol·L~(-1))Zn处理。试验结果表明:过量Zn胁迫严重影响植株生长及Zn的积累与分配,随着Zn处理浓度的升高,八棱海棠幼苗根系中Zn含量显著高于茎和叶;富集系数(BAF)的增大和转运系数(TF)的减小都说明Zn在根系中大量积累,以减轻对地上部的伤害。根据对9个MTP基因的相对表达量以及相关性分析,认为在高Zn胁迫下MTP基因的表达与植株内Zn含量有密切联系。MTP1在茎、叶中显著表达,具有组织特异性。MTPC2-like的相对表达量在4μmol·L~(-1) Zn处理下与根、茎、叶的Zn含量显著正相关,而在较高浓度Zn胁迫下相关性减弱,这说明MTP基因的表达具有浓度效应。
In order to study the mechanism of Zn accumulation, distribution and molecular response in rootstocks of Malus robusta Rehd. Under high Zn stress, the 8-leaf-aged seedlings were used as experimental material to study the effects of different concentrations (4, 25, 50, 100μmol·L -1) Zn treatment. The results showed that excessive Zn stress seriously affected the plant growth and the accumulation and distribution of Zn. With the increase of Zn concentration, the content of Zn in the root system of B. catepitosa seedlings was significantly higher than that of the stem and leaves. The enrichment coefficient (BAF) Both the large and translocation coefficients (TF) indicate that Zn accumulates in the root system in large quantities to mitigate damage to the aerial parts. According to the relative expression of 9 MTP genes and the correlation analysis, it is considered that the expression of MTP gene is closely related to the content of Zn in plants under high Zn stress. MTP1 is highly expressed in stem and leaf and has tissue specificity. The relative expression level of MTPC2-like was significantly and positively correlated with the Zn content of roots, stems and leaves under 4μmol·L -1 Zn treatment, but decreased at higher concentrations of Zn stress, indicating that MTP gene expression Has a concentration effect.