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目的比较2种光源对体外3T3细胞光毒性试验结果的影响。方法参照化学品体外3T3中性红摄取光毒性试验指导原则(OECD 432),采用模拟阳光光源和紫外光源对6种参考物质进行试验。结果 6种参考物质在2种光源照射后其光刺激因子和平均光效应均接近OECD 432中的参考值,各个物质在无光和有光下的IC_(50)值也相近。结论 2种光源对体外3T3细胞光毒性试验结果基本无影响。
Objective To compare the effects of two light sources on the phototoxicity of 3T3 cells in vitro. Methods According to the guidelines of 3T3 neutral red in vitro phototoxicity test of chemicals (OECD 432), six reference materials were tested by simulated sunlight and ultraviolet light sources. Results The results showed that the light stimulating factors and average light effects of 6 reference materials were close to those of OECD 432 after irradiation with 2 kinds of light sources. The values of IC 50 in both light and light were also similar. Conclusion The results of two kinds of light sources on the phototoxicity of 3T3 cells in vitro have no effect.