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目的 建立能进行光学活体分子成像的lewis肺癌 (LLC)移植瘤模型 ,探讨其在观察研究肿瘤的生长、转移、肿瘤血管生成及治疗效果等方面的价值。方法 利用 pLEIN CMV GFP逆转录病毒载体将绿色荧光蛋白 (greenfluorescentprotein ,GFP)报告基因转入小鼠Lewis肺癌 (LLC)肿瘤细胞 ,体外稳定高效表达后 ,种植于小鼠双侧腹股沟 ,在不同时间利用光学活体成像仪成像 ,观察并用病理学证实。结果 所有动物均成功制成荷GFP基因LLC移植瘤模型 ,GFP基因稳定高效表达 ,可见随肿瘤生长 ,绿色荧光团块增大。结论 该模型稳定可靠 ,能够使我们利用光学分子成像的方法 ,实时、无创、连续地观察肿瘤生长 ,为研究肿瘤提供了新的思路和方法。
OBJECTIVE: To establish lewis lung cancer (LLC) xenograft model that can perform molecular imaging in vivo and investigate its value in observing tumor growth, metastasis, tumor angiogenesis and therapeutic effect. Methods Green fluorescent protein (GFP) reporter gene was transfected into mouse Lewis lung cancer (LLC) tumor cells by pLEIN CMV GFP retrovirus vector. After stable and high-expression in vitro, the reporter gene was planted in the bilateral inguinal region of mice and used at different times Optical living body imager imaging, observation and pathology confirmed. Results All animals were successfully established LLC model of GFP-bearing LLC transplanted tumor. The GFP gene was stably and efficiently expressed. As the tumor grew, the green fluorescent mass increased. Conclusion The model is stable and reliable, enabling us to use optical molecular imaging methods to observe tumor growth in real time, noninvasively and continuously, providing new ideas and methods for tumor research.