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目的研究皮肤T细胞淋巴瘤(CTCL)早期诊断。方法利用PCR方法,设计TCRVγ1-8、Vγ9、Jγ1/γ2特异引物,分析24例各类型CTCL,2例可疑CTCL及4例非特异性红皮病患者的33份皮肤和6份淋巴结石蜡标本的TCRγ鄄GR克隆性,30例炎性皮疹石蜡标本为阴性对照。结果39份标本中38份示TCRVγ1-8,37份示Vγ9呈MCGR、BCGR或OCGR。尤其是13例早期蕈样肉芽肿,2例可疑CTCL和4例非特异性红皮病均呈TCRγ鄄GR克隆性扩增带。6例炎性病变标本示TCRγ鄄GR克隆性,提示他们为“克隆性皮炎”,需长期随访。结论用PCR发现早期CTCL的TCRγ鄄GR克隆性,为诊断提供依据。
Objective To study the early diagnosis of cutaneous T-cell lymphoma (CTCL). Methods TCRVγ1-8, Vγ9 and Jγ1 / γ2 specific primers were designed and used to analyze TCRγ in 33 cases of skin and 6 lymph node specimens of 24 cases of CTCL, 2 cases of suspicious CTCL and 4 cases of non-specific erythroderma. GR clonality, 30 cases of inflammatory rash paraffin specimens as a negative control. Results 38 of the 39 samples showed TCRVγ1-8, and 37 showed that Vγ9 was MCGR, BCGR or OCGR. In particular, 13 cases of early mycosis fungoides, 2 cases of suspected CTCL and 4 cases of nonspecific erythroderma showed TCRγ GR clonal amplification band. 6 cases of inflammatory lesions showed TCRγ GR clonality, suggesting that they are “clonogenic dermatitis”, requiring long-term follow-up. Conclusion The clonality of TCRγ-GR in early stage CTCL was detected by PCR, which provided the basis for diagnosis.