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应用聚合酶链反应-限制性片段长度多态性分析法，测定131例健康正常人和131例冠心病患者的载脂蛋白E（ApoE）基因型，并分析该基因对两组受试者的血浆血脂、脂蛋白水平的影响。结果显示ApoE等位基因对血脂具有显著的调节作用，ApoE4等位基因具有升高总胆固醇（TC）、低密度脂蛋白胆固醇（LDL－C）的作用；而ApoE2等位基因则有相反的作用，ApoeE4平均升高TC约0．32～0．58mmol／L；ApoE2平均降低TC约0．34～0．61mmol／L，提示ApoE等位基因导致个体间血脂水平的明显差异。
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the apolipoprotein E (ApoE) genotypes in 131 healthy controls and 131 coronary heart disease patients. Effect of plasma lipids and lipoprotein levels. The results showed that the ApoE allele had a significant regulatory effect on blood lipids, the ApoE4 allele had the effect of increasing total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C), while the ApoE2 allele had the opposite effect , ApoeE4 average elevated TC about 0.32 ~ 0.58mmol / L; ApoE2 average reduction of TC about 0.34 ~ 0.61mmol / L, suggesting that ApoE allele led to significant differences in the level of blood lipid among individuals.