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用体外短期液体培养技术，观察ＲＡ和不同来源的造血调控因子对ＨＬ－６０细胞增殖与分化的影响。以生长曲线和倍增时间作为观察各种试剂对ＨＬ－６０细胞增殖作用的指标；以ＮＢＴ还原反应和细胞形态变化作为分化成熟的指标。结果是ＲＡ、ＰＨＡ－ＬＣＭ单用时对ＨＬ－６０细胞均有抑制增殖和诱导分化作用，ＰＷＭ－ＳＣＭ、ＬＰ３－ＣＭ仅部分抑制增殖，而ＦＧＦ、Ｈｕ－ＣＳＦ和ＥＰＯ等无效。各种造血生长因子分别与ＲＡ合用时，能加强ＲＡ对ＨＬ－６０细胞的增殖抑制和诱导分化作用的有ＦＣＦ、ＰＷＭ－ＳＣＭ、ＰＨＡ－ＬＣＭ和Ｈｕ－ＣＳＦ等；ＬＰ３－ＣＭ仅增强抑制增殖：ＥＰＯ未显示作用。
Using in vitro short-term liquid culture techniques, the effects of RA and hematopoietic regulators from different sources on the proliferation and differentiation of HL-60 cells were observed. The growth curve and doubling time were used as indicators of the proliferation of HL-60 cells observed with various reagents, and NBT reduction and cell morphology were used as indicators of differentiation maturation. As a result, both RA and PHA-LCM inhibited proliferation and induced differentiation of HL-60 cells when used alone. PWM-SCM and LP3-CM only partially inhibited proliferation, whereas FGF, Hu-CSF and EPO were ineffective. When hematopoietic growth factors are combined with RA, they can enhance the inhibition of proliferation and induction of differentiation of HL-60 cells by FCF, PWM-SCM, PHA-LCM and Hu-CSF; LP3-CM only enhances proliferation inhibition. : EPO does not show effect.