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采用苏云金芽孢杆菌制剂美国标准品:Bacillus thuringiensis subsp. kurstaki HD-1-S-1980(H_(3a3b))对不同亚种制剂:Bacillus thuringiensis subsp. dendrolimus U菌株(H_(4a4b))和Bacillus thuringiensis subsp. galleriae C88菌株(H_5a5b))进行了毒力效价的研究。以U菌株制备的标样与美国标准品对不同试虫进行了比较。生物测定的结果表明,U菌株标样的效价分别为18666.6IU/mg(试虫为马尾松毛虫Dendrolimus punctatus 2龄幼虫)、22956.5IU/mg(试虫为小菜蛾Plutella xylostella 2龄幼虫),均高于美国标样16000IU/mg的水平。其敏感度为小菜蛾大于马尾松毛虫。以U菌株和C88菌株生产的中试产品用美国标准品进行毒力测定,试虫为马尾松毛虫,其结果表明,U菌株产品毒力效价为36444.4IU/mg;C88菌株产品为28521.7IU/mg,两者均明显高于美国标准品。试验结果还表明,采用美国标准品及马尾松毛虫和小菜蛾为试虫,来校正我国目前现有产品的毒效是可行的,用作者编定的BASIC计算程序,以微机进行统计分析,比用计算器进行结果运算准确、迅速。
Bacillus thuringiensis subsp. Dendrolimus U (H_ (4a4b)) and Bacillus thuringiensis subsp (Bacillus thuringiensis subsp. Kurstaki HD-1-S-1980 galleriae C88 strain (H_5a5b)). The standard strains prepared from strain U were compared with American standards for different test insects. Bioassay results showed that the titer of the U strain standard samples were 18666.6 IU / mg (2nd instar larvae of Dendrolimus punctatus), 22956.5 IU / mg (2nd instar larvae of Plutella xylostella) Are higher than the US standard 16000IU / mg level. Its sensitivity to Plutella xylostella is greater than that of Dendrolimus punctatus. The virulence of the pilot product produced by U strain and C88 strain was measured by American standards. The test insect was Dendrolimus punctatus. The results showed that the virulence titer of strain U was 36444.4 IU / mg and that of C88 strain was 28521.7 IU / mg, both significantly higher than the United States standards. The test results also show that the use of the United States standards and the massonoformilla and Plutella xylostella as test insects, to correct the current toxic effects of our products is feasible, with the author of the BASIC calculation program to computer statistical analysis, than Calculate results with the calculator accurate and rapid.