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为从分子遗传水平探讨教练员高脂血症及潜在的动脉粥样硬化（Ａｓ）或冠心病（ＣＨＤ）的病因，我们通过Ｓｏｕｔｈｅｒｎ分子杂交法，用人载脂蛋白Ａ－ＩｃＤＮＡ探针，对５１名优秀运动员出身的国家集训队教练员进行载脂蛋白Ａ－Ｉ基因ＰｓｔＩ位点的限制性片段长度多态性（ＲＦＬＰｓ）分析。杂交结果出现２．２ｋｂ和３．３ｋｂ两条与文献报道近似的杂交带，５１名教练员中Ｐ＿２等位基因（３．３ｋｂ）频率为０．１３。与一文献报道的在中国人群中所作的类似研究比较，Ｐ＿２等位基因频率，教练员接近陈旧性心梗患者（Ｐ＿２频率＝０．２０，Ｐ＞０．０５），而显著高于正常对照人群（Ｐ＿２频率＝０．０２，Ｐ＜０．０１）。提示优秀运动员出身的教练员和／或优秀运动员具有某些遗传特异性，尤其值得重视的是潜在的Ａｓ或ＣＨＤ易感性。本研究中，具有或不具Ｐ＿２等位基因的教练员，ＴＧ、ＴＣ、ＨＤＬ－ｃ、ａｐｏＡ－Ｉ、ａｐｏＢ等五种血脂水平无显著差异。
In order to explore the causes of hyperlipidemia and potential atherosclerosis (As) or coronary heart disease (CHD) in coaches from the perspective of molecular genetic level, we used Southern hybridization with the apolipoprotein A-I cDNA probe A national team coach from a famous athlete performed restriction fragment length polymorphism (RFLPs) analysis of the PstI site of the apolipoprotein A-I gene. The results of hybridization showed two hybrids with 2.2kb and 3.3kb reported in the literature. The frequency of P_2 allele (3.3kb) in 51 coaches was 0.13. Compared with a similar study reported in the Chinese population, P 2 allele frequency was significantly higher in coaches than in elderly patients with chronic MI (P 2 frequency = 0.20, P> 0.05) Population (P_2 frequency = 0.02, P <0.01). It is suggested that coaches and / or elite athletes from elite athletes have some genetic specificity, with particular regard to potential As or CHD susceptibility. In this study, there was no significant difference in the five lipid levels of TG, TC, HDL-c, apoA-I and apoB with or without P 2 allele.