目的 :寻找适合于云南红豆杉愈伤组织生长和形成紫杉醇的培养条件。方法 :对在不同光照条件下和不同培养基上的愈伤组织进行生长分析和紫杉醇含量测定。结果与结论 :黑暗、0 .1mg·L-1BAP和 1.0mg·L-12 ,4 D的激素组合 ,B5,DCR或 6 ,7 V基本培养基有利于云南红豆杉愈伤组织生长和形成紫杉醇 ;培养基中NO3 -浓度高有利于愈伤组织的生长 ,NH+ 4浓度高抑制愈伤组织的生长 ,但显著提高紫杉醇含量。 Objective: To find suitable culture conditions for callus growth and paclitaxel formation in Yunnan. Methods: Callus growth assay and paclitaxel assay were performed on callus under different light conditions and on different media. RESULTS AND CONCLUSION: The combination of dark, 0 .1 mg · L-1BAP and 1.0 mg · L-12, 4 D hormone combinations, B5, DCR or 6, 7 V minimal medium was beneficial to callus growth and the formation of paclitaxel . The high concentration of NO3 - in culture medium was beneficial to the growth of callus. The high concentration of NH + 4 inhibited the growth of callus, but significantly increased the content of paclitaxel.