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目的 对三种常用超广谱 β -内酰胺酶 (ESBLs)测定方法进行评价. 方法 采用纸片扩散法、双纸片增效试验、双纸片协同试验三种方法测定 30株ESBLs阳性和 5 0株ESBLs阴性的革兰氏阴性杆菌及临床分离的 6 5株革兰氏阴性杆菌产ESBLs情况.结果 纸片扩散法、双纸片增效试验、双纸片协同试验三种方法敏感性分别为 93.3%、86 .7%、93.3%,特异性分别为 90 .0%、10 0 .0%、10 0 .0%,临床分离菌株ESBLs检出率为 15 .4%.结论 三种方法均可选用 ,但以纸片协同试验测定简便 ,敏感性、特异性均好 ,更适合各级医院常规应用.“,”Objcetive To evaluate three routine methods for detection of extended-spectrum β-lactamases(ESBLs). Methods Three routine methods disk test, double-disk enhancement test and double-disc synergy test were applied to detect 30 ESBLs-producing and 50 non-ESBLs producing strains of Gram negative bacilli and also to determine the situation of strains producing ESBLs among 65 strains of Gram negative bacilli isoluted from clinical samples.Results The sensitivity of three routine methods disk test a double disk enhanmcement test and double-disk synergy test, was found to be 93.3%,86.7% and 93.3% respectively and their specificity 90.0%,100.0% and 100.0% respectively. The detectable rate of ESBLs in isolates of Gram negative bacilli was 15.4%.Conclusions Three routine methods are all suitable for detecting ESBLs, but the double-disk synergy test is the most sensitive and reliable among three routine methods for the detection of ESBLs in Gram negative bacilli, and more suitable for screening strains producing ESBLs routinely in all hospitals.