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目的观察成纤维细胞生长因子对培养兔关节软骨细胞转化生长因子－β１的表达。方法培养１月龄新西兰兔的关节软骨细胞，在每次换液时加入成纤维细胞生长因子１００ｎｇ／ｍｌ，铺满后收集细胞，作关节软骨细胞转化生长因子－β１常规及电镜免疫组织化学研究。结果光镜下实验组细胞明显呈棕色，电镜下细胞膜表面有明显的胶金颗粒粘附。结论成纤维细胞生长因子能促进关节软骨细胞转化生长因子－β１的表达。
Objective To observe the expression of transforming growth factor-β1 in cultured rabbit articular chondrocytes with fibroblast growth factor. Methods Articular chondrocytes of 1-month-old New Zealand rabbits were cultured and perfused with 100ng / ml fibroblast growth factor after confluence. Cells were collected after confluence and routinely and electron microscopy immunohistochemically studied on the expression of transforming growth factor-β1 in articular chondrocytes . Results The cells in the experimental group under light microscope showed a clear brown color. The surface of the cell membrane under the electron microscope showed obvious adhesion of gold particles. Conclusion Fibroblast growth factor can promote the expression of transforming growth factor-β1 in articular chondrocytes.