【目的】比较水稻矮秆多分蘖突变体与野生型材料间的植物激素含量差异,并分析不同生长调节剂处理对其株高、分蘖的影响,探析植物激素在突变体形态发育过程中的生理功能。【方法】以T-DNA插入引起的矮秆多分蘖突变体(CA648)及对照中花11(ZH11)为供试材料,采用高效液相色谱法(HPLC)分析三叶期、五叶期、分蘖盛期、拔节孕穗期的根、假茎(或茎)、叶中的赤霉素(GA1,GA4)、生长素(IAA)、玉米素(Z)和脱落酸(ABA)的含量,进行CA648与ZH11在不同生长发育时期的不同器官中的植物激素含量差异比较分析;利用液相色谱-串联质谱联用法(HPLC-MS/MS)测定拔节孕穗期根、基部、茎秆及叶片中的IAA、Z及玉米素核苷(ZR)的含量并进行根、茎、叶等不同器官中IAA与Z+ZR含量间的比值差异分析;在CA648与ZH11的不同生长发育时期喷施不同浓度的GA3、IAA、Z及多效唑处理后统计株高、分蘖的变化特性,并比较分析CA648与ZH11对不同生长调节剂处理的反应敏感性差异。【结果】不同生长发育时期的CA648与ZH11的不同器官中的植物激素存在不同变化趋势。GA1+4含量变化情况为在三叶期和五叶期的叶片中两者相差不大,而根和假茎中的含量则是CA648高于ZH11;从五叶期至拔节期,随着生育期延长,ZH11的假茎和根中GA1+4含量升高而叶片中含量降低,但CA648却表现出相反的变化趋势,假茎和根中的GA1+4含量降低但叶片中含量却升高。IAA含量变化情况为五叶期、分蘖盛期时CA648叶片中的IAA含量低于ZH11,而假茎中的IAA含量却高于ZH11;拔节期时,CA648的根、茎、叶中IAA含量均与ZH11对应器官中的IAA含量相差不大。Z、ZR含量变化情况为三叶期CA648的根与假茎中的Z含量都低于ZH11;在拔节期,分蘖部位的基部和茎中的Z+ZR含量为CA648高于ZH11,而叶片中的Z+ZR含量却是CA648略低于ZH11。ABA含量变化情况为在三叶期、五叶期时CA648的ABA含量高于ZH11,而拔节期却显著低于ZH11。IAA与Z+ZR的比值情况为在顶叶、茎秆中是CA648大于ZH11,而在基部和根中则是CA648中的比值小于ZH11。不同浓度GA3、IAA、Z和多效唑喷施处理表明CA648的株高易受生长调节剂的影响。与喷水对照相比,喷施GA3、IAA、Z均能增加CA648的株高,其敏感性依次为GA3处理>IAA处理>Z处理;喷施多效唑后CA648与ZH11的株高均明显降低;虽然CA648与ZH11的株高均受GA3、多效唑等生长调节剂的影响,但CA648更敏感,当喷施的GA3浓度≥288μmol·L-1时,CA648的株高能够达到或超过ZH11的株高;与株高易受生长调节剂的影响不同,CA648的多分蘖特性几乎不受长调节剂处理的影响,无论是否喷施生长调节剂,CA648的分蘖数均远远多于ZH11。【结论】与对照相比,CA648体内的GA1、GA4、IAA、Z、ZR含量及其IAA与Z+ZR的比值有利于矮秆多分蘖性状的形成,即植物激素含量与比例能够反映CA648的生理特性;生长调节剂对CA648的分蘖与株高具有不同调控效果,喷施GA3、IAA、Z和多效唑能够显著改变株高,但其多分蘖特性不受株高改变的影响。 【Objective】 The objective of this study is to compare the differences in plant hormone content between rice tillering mutant and wild-type material, and to analyze the effects of different growth regulators on their plant height and tillering, and to explore the physiological roles of plant hormones in the morphological development of mutants Features. 【Method】 The short tiller mutant (CA648) and control Zhonghua 11 (ZH11) induced by T-DNA insertion were used as experimental materials. The contents of three-leaf stage, five-leaf stage, The contents of gibberellin (GA1, GA4), auxin (IAA), zeatin (Z) and abscisic acid (ABA) in the roots, stems and stems at the time of tillering, The content of phytohormones in different organs of different growth stages of CA648 and ZH11 were compared and analyzed. The contents of plant hormones in root, base, stem and leaves at jointing booting stage were determined by liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) IAA, Z and zeatin riboside (ZR) content in different organs of roots, stems, leaves and other organs IAA and Z + ZR content ratio between the differences analysis; CA648 and ZH11 different growth and development of spraying different concentrations of GA3, IAA, Z and paclobutrazol treatment of plant height, tillering characteristics, and comparative analysis of CA648 and ZH11 on different growth regulators response to sensitivity differences. 【Result】 There were different trends of plant hormones in different organs of CA648 and ZH11 at different growth and development stages. The content of GA1 + 4 changed little in the leaves of the three-leaf stage and the five-leaf stage, while the contents of the root and the pseudostem were CA648 higher than ZH11. From the five-leaf stage to the jointing stage, Prolonged, the content of GA1 + 4 in pseudostem and root of ZH11 increased while the content of leaf decreased, but CA648 showed the opposite trend. The content of GA1 + 4 in pseudostem and root decreased but the content in leaf increased . The content of IAA in leaves of five-leaf stage was the five-leaf stage. The content of IAA in the leaves of CA648 at the tillering stage was lower than that of ZH11, while the content of IAA in pseudostem was higher than that of ZH11. IAA content in roots, stems and leaves of CA648 IA11 and ZH11 corresponding organs in the same or less. The content of Z and ZR in roots and stems of CA648 in the three-leaf stage was lower than that in ZH11. At the jointing stage, the content of Z + ZR in the roots and stems of the tillering parts was higher than ZH11 in CA648, The Z + ZR content is slightly lower than the CA648 ZH11. ABA content in the three-leaf stage, five-leaf stage CA648 ABA content higher than ZH11, but significantly lower than join ZH11. The ratio of IAA to Z + ZR was CA648 greater than ZH11 in the parietal lobe, whereas in the base and root, the ratio of CA648 was less than ZH11. Different concentrations of GA3, IAA, Z and paclobutrazol spray treatment showed that the plant height of CA648 susceptible to growth regulator. The plant height of CA648 could be increased by spraying GA3, IAA and Z compared with that of water spray control. The sensitivity of GA3, IAA and Z treatments was GA3> IAA> Z, and the plant height of CA648 and ZH11 were significantly decreased after spraying paclobutrazol. Although the plant height of CA648 and ZH11 were all affected by growth regulators such as GA3 and paclobutrazol, CA648 was more sensitive. When the GA3 concentration was 288μmol·L-1, the plant height of CA648 could reach or exceed the plant height of ZH11 In contrast to the susceptibility of plant height to growth regulators, the multi-tillering trait of CA648 was almost unaffected by the long-conditioning regulators. The number of tillering of CA648 was much higher than that of ZH11 with or without growth regulators. 【Conclusion】 Compared with the control, the content of GA1, GA4, IAA, Z, ZR and the ratio of IAA to Z + ZR in CA648 are favorable to the formation of dwarfing and tillering traits. That is to say, the content and proportion of plant hormone can reflect the ratio of CA648 The growth regulators had different regulation effects on the tillering and plant height of CA648. The spraying of GA3, IAA, Z and paclobutrazol significantly changed plant height, but the multi-tillering characteristics were not affected by plant height changes.