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目的:分析SH2-B对结肠癌细胞侵袭迁移的影响,探讨结肠癌转移的分子机制。方法:免疫荧光筛选SH2-B低表达结肠癌细胞,用Lipofectamine 2000TM将pcDNA3.1-SH2-B质粒转染至HT-29细胞,细胞划痕实验分析SH2-B对结肠癌细胞HT-29爬行迁移运动的影响,用Boden Chamber分析SH2-B对结肠癌细胞HT-29侵袭运动的影响。结果:HT-29为SH2-B低表达结肠癌系,基因转染后HT-29细胞表达SH2-B显著增高;细胞划痕研究结果表明,SH2-B转染组、空载体转染组和未转染母细胞组迁移细胞数倒置显微镜10个视野分别为867±187、349±121和279±158,SH2-B转染组爬行细胞数显著高于空载体组和未转染细胞组,P均<0.05;Trans-well研究结果显示,转染SH2-B组、空载体转染组和未转染细胞组穿过滤膜细胞数分别为278±107、129±88和112±81,转染SH2-B组透过细胞数显著高于未转染组和空载体转染组,P<0.05。结论:SH2-B可能通过增强结肠癌细胞运动和迁移能力参与结肠癌细胞的侵袭和转移。
Objective: To analyze the effect of SH2-B on the invasion and migration of colon cancer cells and to explore the molecular mechanism of metastasis of colon cancer. Methods: SH2-B low expression colon cancer cells were screened by immunofluorescence. The pcDNA3.1-SH2-B plasmid was transfected into HT-29 cells with Lipofectamine 2000TM. Cell scratch assay was used to analyze the effect of SH2-B on colon cancer cells HT-29 The effect of SH2-B on invasiveness of colon cancer cells HT-29 was analyzed by Boden Chamber. Results: HT-29 was a low expression of SH2-B colon cancer, and the expression of SH2-B in HT-29 cells was significantly increased after transfection. The results of cell scratch assay showed that SH2-B transfection group, The number of migrating cells in untransfected blastocysts was 867 ± 187,349 ± 121 and 279 ± 158, respectively. The number of crawling cells in SH2-B transfected group was significantly higher than that in empty vector group and untransfected group, P <0.05, respectively. The results of Trans-well assay showed that the number of transfected cells in SH2-B group was 278 ± 107,129 ± 88 and 112 ± 81 Cell number of SH2-B transfected cells was significantly higher than that of untransfected cells and empty vector transfected cells (P <0.05). Conclusion: SH2-B may participate in the invasion and metastasis of colon cancer cells by enhancing the ability of colon cancer cells to move and migrate.