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目的:对急性白血病的免疫分型进行分析探讨,为今后的临床工作提供有价值的参考信息。方法:以符合临床诊断标准的成人急性白血病患者182例作为研究对象,包括有急性髓系白血病(AML)114例,其中合并淋系抗原的急性髓系白血病22例,急性淋巴细胞白血病(ALL)68例(急性B细胞白血病54例,急性T细胞白血病14例),其中合并髓系抗原表达的淋巴细胞白血病患者6例。对各组患者采取流式细胞术展开免疫分型检测,并对检测结果进行对比分析。结果:AML患者主要表达CD13、CD33、CD34、HLA-DR、CD117抗原;68例ALL患者抗原表达阳性率从高到底逐渐是:HLA-DR 79.41%,CD10 64.71%,CD19 60.29%,CD34 42.65%,CD7 29.41%。B急淋一般表达CD19,CD10等,而T急淋一般表达CD3,CD7等。合并淋系抗原表达的AML患者,临床治疗中,完全缓解率低于无淋系抗原表达的AML患者(P<0.05);合并髓系抗原表达的ALL患者化疗后,完全缓解率与无髓系抗原表达的ALL患者比较,差异无统计学意义(P>0.05)。结论:对成人急性白血病进行免疫分型,能够对正常骨髓细胞的影响予以排除,对患者的免疫学分型进行准确反映,值得关注。
Objective: To analyze and discuss the immunophenotyping of acute leukemia and provide valuable reference information for future clinical work. Methods: A total of 182 adult patients with acute leukemia were enrolled in this study, including 114 cases of acute myeloid leukemia (AML), 22 cases of acute myeloid leukemia with lymphocytic leukemia, 20 cases of acute lymphoblastic leukemia (ALL) 68 cases (acute B cell leukemia in 54 cases, acute T cell leukemia in 14 cases), which merged myeloid antigen expression in 6 patients with lymphocytic leukemia. The patients in each group were detected by flow cytometry immunophenotyping, and the test results were compared. Results: The positive expression rates of CD13, CD33, CD34, HLA-DR and CD117 in AML patients were 68.4%, 79.61%, 64.71%, 60.69% , CD7 29.41%. B acute lymphoid manifestations of CD19, CD10, etc., and T acute lymphoblastoid express CD3, CD7 and so on. In patients with AML with lymphoid antigen expression, the complete remission rate was lower in clinical treatment than in AML patients without lymphoid antigen expression (P <0.05). After chemotherapy, the complete remission rate in patients with ALL with myeloid antigen expression was significantly higher than that in patients without myeloid The antigen expression of ALL patients, the difference was not statistically significant (P> 0.05). Conclusion: The immunophenotyping of adult acute leukemia can exclude the influence of normal bone marrow cells. It is worth to pay attention to accurately reflect the immunological typing of patients.