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目的：运用脑缺血再灌注模型，探索血小板活化因子及自由基在缺血性脑损伤中的地位及意义。方法：分别应用放免法及TBA法测定大鼠海马组织中PAF与LPO含量。结果：缺血20min后再灌注0min组及60min组PAF含量均显著高于假手术对照组，而再灌注240min组与假手术对照组比较已无显著差异。相应LPO含量早期随再灌注时间延长逐渐升高。结论：PAF可能主要参与脑缺血再灌注缺血期的病理损伤机制；而自由基的脂质过氧化作用增强发生在再灌注期。
Objective: To explore the status and significance of platelet-activating factor and free radicals in ischemic brain injury by using cerebral ischemia-reperfusion model. Methods: The levels of PAF and LPO in hippocampus of rats were determined by radioimmunoassay and TBA respectively. Results: PAF content in 0 min group and 60 min group after reperfusion for 20 min was significantly higher than that in sham-operation control group, but there was no significant difference between reperfusion 240 min group and sham operation control group. The corresponding LPO content gradually increased with the prolongation of reperfusion time. CONCLUSION: PAF may be involved in the pathogenesis of ischemia-reperfusion injury and the lipid peroxidation of free radicals may occur during reperfusion.