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将来自10名正常男性的精液标本进行冷冻保存,复温后电镜观察.结果示冷冻精子复温后精子超微结构发生不同程度的改变,其主要改变为精子顶体肿胀,顶体内、外膜分离,外膜形成波浪状皱褶,有的核局部出现凹陷.线粒体基质密度降低、结构模糊等.但精子基本结构保持完整.提示冷冻精子超微结构改变的主要原因是在冷冻过程中,由于冰晶形成的影响.水分内移.细胞肿胀,因而造成其活动率的明显下降.但是并不影响授精能力。
The sperm samples from 10 normal men were cryopreserved and rewarmed for electron microscopy.The results showed that ultrastructures of sperm were changed to varying degrees after rewarming, the main changes were sperm acrosome swelling, acrosome, adventitia Separation, the outer membrane of the formation of wavy folds, and some nuclear depression. Mitochondrial matrix density decreased, fuzzy structure, etc. However, the basic structure of sperm intact. Tip frozen sperm ultrastructure of the main changes in the freezing process, due to The formation of ice crystals. Water migration in. Cell swelling, resulting in a significant decline in the activity rate, but does not affect the ability of insemination.