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为探索糖尿病大血管并发症的发病机理,应用人生长激素对伴和不伴高脂血清培养的兔主动脉平滑肌细胞进行细胞动力学(以 DNA 增殖率为指标)和细胞形态学(光镜及透射电镜)检测。结果显示:人生长激素对平滑肌细胞非高脂血清组的增殖有明显抑制作用,且DNA 增殖率与生长激素浓度之间呈明显负相关(r=-0.49,P<0.01),相对应的,形态学上表现为平滑肌细胞不同程度蜕变。但高脂情况下,生长激素浓度为1～100ng/ml 时,则 DNA合成率明显上升,且细胞结构中细胞器增殖。在此时有特殊意义的是,平滑肌细胞间前胶原纤维大量出现。但当生长激素浓度达1000ng/ml 时,高脂血清组则呈细胞生长抑制作用。因此,本文结果提示:HGH1～100ng/ml 时,能促进动脉粥样硬化的形成。
In order to explore the pathogenesis of diabetic macrovascular complications, the effects of human growth hormone on rabbit aortic smooth muscle cells cultured with or without high-fat serum were investigated by cell kinetics (DNA proliferation rate as an index) and cell morphology Transmission Electron Microscopy). The results showed that human growth hormone significantly inhibited the proliferation of smooth muscle cells in non-hyperlipidemic serum, and there was a negative correlation between DNA growth rate and growth hormone concentration (r = -0.49, P <0.01). Correspondingly, Morphological manifestations of smooth muscle cells varying degrees of metamorphosis. However, in the case of high fat, when the concentration of growth hormone is 1 ~ 100ng / ml, the DNA synthesis rate rises obviously, and the organelles proliferate in the cell structure. Of special significance at this point is the presence of a large amount of procollagen fibers between smooth muscle cells. However, when the growth hormone concentration reached 1000ng / ml, the hyperlipidemia group showed a cell growth inhibitory effect. Therefore, the results suggest that: HGH1 ~ 100ng / ml, can promote the formation of atherosclerosis.