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NR2B subunits are involved in regulating aging,in particular,age-related learning and memory deficits.We examined 19-month-old NR2B transgenic mice and their littermate controls.First,we detected expression of the NR2B subunit gene,Grin2b,in the neocortex of transgenic mice using real-time PCR.Next,we used microarrays to examine differences in neocortical gene expression.Pathway and signal-net analyses identified multiple pathways altered in the transgenic mice,including the P53,Jak-STAT,Wnt,and Notch pathways,as well as regulation of the actin cytoskeleton and neuroactive ligand-receptor interactions.Further signal-net analysis highlighted the P53 and insulin-like growth factor pathways as key regulatory pathways.Our results provide new insight into understanding the molecular mechanisms of NR2B regulated age-related memory storage,normal organismal aging and age-related disease.
NR2B subunits are involved in regulating aging, in particular, age-related learning and memory deficits. We examined 19-month-old NR2B transgenic mice and their littermate controls. First, we detected expression of the NR2B subunit gene, Grin2b, in the neocortex of transgenic mice using real-time PCR .Next, we used microarrays to examine differences in neocortical gene expression. Pathway and signal-net analyzes identified multiple pathways altered in the transgenic mice, including the P53, Jak-STAT, Wnt, and Notch pathways , as well as regulation of the actin cytoskeleton and neuroactive ligand-receptor interactions. Further signal-net analysis highlighted the P53 and insulin-like growth factor pathways as key regulatory pathways. Our results provide new insights into understanding the molecular mechanisms of NR2B regulated age -related memory storage, normal organismal aging and age-related disease.