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作者构建了编码HBsAg的表达质粒pCI/S并建立了能稳定表达HBsAg的H-2~d肥大细胞瘤细胞系P815/S和B淋巴瘤细胞系A20/S。将10或100μg pCI/S分别注射Ba1b/c（H-2~d）小鼠的胫骨前肌（im）或四头肌、尾基部的皮下组织（sc）、腹腔（ip）、尾静脉（iv）。对照组注射等量的空白载体质粒
The authors constructed the expression plasmid pCI / S encoding HBsAg and established the H8 cell line of H-2-d mast cell tumor P815 / S and the B lymphoma cell line A20 / S which can stably express HBsAg. The tibial anterior or quadriceps of Ba1b / c (H-2 ~ d) mice were inoculated with pCI / S at 10 or 100μg, the scalp at the base of the tail, the ip and tail vein iv). The control group was injected with the same amount of blank vector plasmid