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采用一锅合成法制备了新型的具有大比表面积的花状铂纳米颗粒(PtNFs),并构建了一个高灵敏电致化学发光(ECL)免疫传感器用于检测载脂蛋白A1(Apo-A1).该Pt NFs用于吸附二抗(anti-Apo-A1),并用葡糖糖氧化酶(GOD)封闭其表面的非特异性位点,最终制备了PtNFs@anti-Apo-A1@GOD信号探针.当Apo-A1存在时,通过夹心免疫反应将制备的信号探针捕获于电极表面,并将所制得的电极置于含有葡萄糖的过硫酸根底液中检测.GOD催化葡萄糖产生H_2O_2,H_2O_2在Pt NFs的催化下分解并在电极表面原位产生O2,所产生的O2能够催化过硫酸根-氧气体系的电致化学发光反应,放大发光信号,提高检测灵敏度.该传感器在0.1 ng·m L~(-1)~100 ng·m L~(-1)范围内对Apo-A1有良好的线性响应,检测下限达到0.03 ng·m L~(-1),有望应用于临床分析诊断.
A new type of flower-like platinum nanoparticles (PtNFs) with large specific surface area was prepared by a one-pot synthesis method and a highly sensitive chemiluminescence (ECL) immunosensor was designed to detect apolipoprotein A1 (Apo-A1) The Pt NFs were used to adsorb the secondary antibody (anti-Apo-A1) and the nonspecific sites on the surface were blocked with glucose oxidase (GOD). Finally, PtNFs @ anti-Apo-A1 @ GOD signal probe When Apo-A1 was present, the prepared signal probe was captured on the electrode surface by sandwich immunoassay, and the prepared electrode was detected in the persulfate containing glucose solution.GOD catalyzed the glucose production of H 2 O 2 and H 2 O 2 The catalytic decomposition of Pt NFs and the in-situ generation of O2 on the surface of the electrode catalyzed the electro-chemiluminescence reaction of the persulfate-oxygen system to amplify the luminescence signal and improve the detection sensitivity.The sensor was operated at 0.1 ng · m L Apo-A1 had a good linear response in the range of ~ (-1) ~ 100 ng · m L ~ (-1) with the detection limit of 0.03 ng · m L ~ (-1), which is expected to be used in clinical diagnosis.