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目的探讨接种不同数量的VX2瘤株活细胞的成瘤率、成瘤时间。方法制备VX2细胞匀浆,并计数活细胞浓度。将新西兰大兔分为三组(每组10只):A组(低细胞数组):活细胞数1×104;B组(中细胞数组):活细胞数为1×105;C组(多细胞数组):活细胞数1×106。分别于接种后5d、10d、14d、21d、28d做B超观察成瘤情况及瘤体的大小、形态、转移等情况。结果A组:于接种后5d、10d、14d、21d、28d分别作腹部B超未见肝内肿瘤生长(0/10);B组:于接种后第10d(1/10)和第14d(8/10),B超显示肝内接种部位可见体积(0.53±0.02)cm3低回声区,有声晕,血供丰富;1只于接种后5d、10d、14d、21d、28d分别作腹部B超未见肝内肿瘤生长(1/10)。平均成瘤时间为(13.5±0.18)天。成瘤率为90%。C组:于接种后第5d(1/10)、第10d(9/10),B超显示肝内接种部位可见体积为(0.67±0.03)cm3低回声区,血供丰富,成瘤率为100%,平均成瘤时间为(9.5±1.33)天。A与B组及A与C组的成瘤率在统计学上有显著差异(χ12=16.36,P1<0.05;χ22=20.0,P2<0.05),B与C组的成瘤率在统计学上无显著性差异(χ2=1.046,P>.0.05)。B组与C组成瘤后体积及肿瘤生长情况无显著差异。结论VX2细胞株是制作兔移植性肝癌模型较好的瘤株,创建模型的成功率及成瘤时间与接种的活细胞数有关,接种活细胞数>1×105则其成瘤率可达90%~100%。VX2活细胞多比活细胞少的成瘤时间早,一般成瘤时间在9天以上。
Objective To investigate the tumorigenic rate and tumorigenesis time of live VECs inoculated with different numbers of VX2 tumor cells. Methods VX2 cell homogenates were prepared and viable cell concentrations were counted. The New Zealand rabbits were divided into three groups (10 in each group): Group A (low cell array): 1 × 104 viable cells; group B (medium cell array): 1 × 105 viable cells; Cell array): live cell number 1 × 106. The tumor formation, size, shape and metastasis of B were observed at 5 days, 10 days, 14 days, 21 days and 28 days after inoculation. Results In group A, intrahepatic tumor growth was not observed in abdominal ultrasonography at 5d, 10d, 14d, 21d and 28d after inoculation (0/10). In group B, intrahepatic tumor growth was observed at 10d (1/10) and 14d B ultrasound showed intrahepatic inoculation site visible volume (0.53 ± 0.02) cm3 hypoechoic area, a sound halo, rich in blood supply; one after inoculation 5d, 10d, 14d, 21d, 28d were made for abdominal B- No intrahepatic tumor growth (1/10). The mean tumor formation time was (13.5 ± 0.18) days. The tumor formation rate was 90%. Group C: At the 5th day (1/10), the 10th day (9/10) after the inoculation, the ultrasonography showed that the intraepithelial inoculation site was (0.67 ± 0.03) cm3 hypoechoic area with abundant blood supply and the tumorigenic rate was 100%, the average tumor formation time was (9.5 ± 1.33) days. The tumorigenic rates of A and B groups and A and C groups were statistically significant (χ12 = 16.36, P1 <0.05; χ22 = 20.0, P2 <0.05). The tumor formation rates of group B and C were statistically No significant difference (χ2 = 1.046, P> 0.05). There was no significant difference in tumor volume and tumor volume between group B and group C. Conclusions The VX2 cell line is a good tumor model for rabbit liver transplantation. The success rate and tumorigenesis time of the established model are related to the number of viable cells inoculated, and the viable rate of tumor cells up to 90% % ~ 100%. VX2 living cells than living cells less tumor formation time, the general tumor formation time in more than 9 days.