论文部分内容阅读
将实验大鼠肝脏接通闭合灌注装置,连续灌注120 min,灌注液经门静脉从下腔静脉流出。70 ml灌注液,含199培养液45％,聚葡糖40％,洗过红细胞块5％和自体血10％。入肝前的灌注液中,直接加2.7mg(第1批实验)和13.5mg(第2批实验)的《菏花牌》合成洗衣粉(CMC)水溶液。剂量分别为0.1和0.5 LD_(50),对照大鼠的灌注液不加CMC液。灌注开始经15,30,60,90和120 min,分别取灌注液测总脂、胆固醇及其脂类、磷脂、甘油三酯等。肝功能评价指标为尿素、pH值、蛋白含量和红细胞计数等。对照大鼠灌注120min,灌注
The experimental rat liver connected closed perfusion device, continuous perfusion 120 min, perfusion fluid through the portal vein from the inferior vena cava outflow. 70 ml of perfusate containing 199% of culture medium 45%, 40% of polyglucose, washed red blood cell block 5% and autologous blood 10%. Pre-heparin perfusate, directly add 2.7mg (first batch of experiments) and 13.5mg (second batch of experiments) “He Hua” synthetic detergent (CMC) aqueous solution. The doses were 0.1 and 0.5 LD_ (50) respectively. The control rats did not receive CMC solution. Perfusion began after 15, 30, 60, 90 and 120 min, were measured perfusion fluid total fat, cholesterol and lipid, phospholipids, triglycerides and so on. Liver function evaluation indicators for urea, pH, protein content and red blood cell count. Control rats were perfused for 120 min and perfused