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用组织培养方法筛选获得羊草（Ａｖｅｕｒｏｌｅｐｉｄｉｕｍｃｈｉｎｅｎｓｅ（Ｔｒｉｎ．）Ｋｉｔａｇ．）抗羟脯氨酸（ＨＹＰ）变异系ＨＲ２０一８。该变异系统细胞内游离氨基酸和蛋白质中氨基酸含量均发生了较大的变化，与供体对照比较，分别提高２．３５倍和１．４０倍。其中的脯氨酸组分在变异系体内表现异常增加，游离和蛋白质中的脯氨酸组分分别提高６．６倍和３．０倍，对脯氨酸合成途径中的γ一谷氨酸激酶的特性分析表明，变异系ＨＲ２０一８细胞内γ一谷氨酸激酶的活性比供体提高２．５倍。
The tissue culture method was used to screen the mutant strain HY20 of Aveneolepidium chinense (Trin.) Kitag. The contents of amino acids and proteins in the intracellular free amino acids of the mutant system all changed greatly, which were 2.35 times and 1.40 times higher than that of donor control respectively. Among them, the proline component showed an abnormal increase in the variation system, and the contents of free amino acid and proline in the protein increased 6.6 times and 3.0 times, respectively, and the activity of proline synthesis in γ-glutamic acid The characterization of kinases showed that the activity of γ-glutamate kinase in mutant strain HR20-8 was 2.5 times higher than that of donor.