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为了解在实际检测条件下，混合血清法筛检丙型肝炎抗体的效果，对１８７５例献血员进行了前瞻性研究。在单个血清检测结果未知的条件下，将每５个血清混合，使用酶免疫法检测丙型肝炎抗体。在检测中保持混合血清中每个标本的稀释度与单个血清检验法相同。结果显示：本次抗－ＨＣＶ筛检的血清阳性率为２．２４％，以单个血清检验为对照，混合血清法的灵敏度为１００％，特异度为９９．２％；阴性混合标本的ＯＤ／ＣＯ值呈正偏态分布，当混合标本中含有高ＯＤ值阴性标本时，可产生假阳性结果。收益分析发现：应用混合血清法筛检抗－ＨＣＶ可节省６９．３３％的检测费用。
In order to understand the effect of screening hepatitis C antibodies by hybrid serum method under the actual test conditions, 1875 blood donors were prospectively studied. In a single serum test results unknown conditions, every 5 serums were mixed, the use of enzyme immunoassay for detecting hepatitis C antibodies. The dilution of each specimen in the pooled serum during the assay is the same as for a single serum test. The results showed that the seroprevalence of this anti-HCV screening was 2.24%, and the single serum test was used as the control. The sensitivity and specificity of the mixed serum method were 100% and 99.2% respectively. The OD / CO values showed a positive skewed distribution, when the mixed specimens contain high OD negative specimens, can produce false positive results. Revenue analysis showed that the screening of anti-HCV using mixed serum method can save 69.33% of the testing costs.